In this course the student will learn to design their primers or initiators, in addition to reviewing concepts about molecular weights in gels as well as the use of bioinformatics tools for design and analysis.

TEMARY

DAY 1

A PDF MANUAL OF PCR

BIOCHEMISTRY OF NUCLEIC ACIDS

CENTRAL DOGMA OF MOLECULAR BIOLOGY

ELECTROPHORESIS IN AGAROSE AND POLYACRYLAMIDE GELS

DAY 2.

GENERAL PRINCIPLES OF THE POLYMERASE CHAIN REACTION

GENERAL PRINCIPLES OF THE DESIGN OF PRIMERS OR INCIATORS

INITIATOR MANUAL DESIGN

PRIMERS DESIGN TO AMPLIFY DNA

VIRTUAL PRACTICE WITH THE NCBI AND BLAST

DAY 3

THE COMPONENTS OF THE PCR

PRACTICAL PRIMER DESIGN USING THE BETA GLOBIN, ACTIN AND ABCB5 GENE

PRIMERS DESIGN TO AMPLIFY cDNA and RETRO TRANSCRIPT

PRIMERS DESIGN WITH PROGRAM

DAY 4

WORKSHOP WITH RESULTS

ANALYSIS AND REPORT OF PCR AMPLIFICATIONS OR AMPLICONS

GENOMIC DNA AMPLIFICATION

AMPLIFICATION TYPE RT-PCR

REAL TIME PCR

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Do you want to know more about the agenda? Download the PDF version