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Course: Design of Primers (initiators)

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Duration: 4 classes 4:00 p.m. to 6:00 p.m.

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Masterclass (webinar) GRATIS: Diciembre 1, 2024

Registro: https://www.pharbiois.com/contacto

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Course start: February 7-10, 2022

Validez oficial/curricular de la red SEP-CONOCER EC0301

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Mode: Online: ZOOM

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Spanish Language

about the course

In this course the student will learn to design their primers or initiators, in addition to reviewing concepts about molecular weights in gels as well as the use of bioinformatics tools for design and analysis.

 

TEMARY

DAY 1

A PDF MANUAL OF PCR

BIOCHEMISTRY OF NUCLEIC ACIDS

CENTRAL DOGMA OF MOLECULAR BIOLOGY

ELECTROPHORESIS IN AGAROSE AND POLYACRYLAMIDE GELS

 

DAY 2.

GENERAL PRINCIPLES OF THE POLYMERASE CHAIN REACTION

GENERAL PRINCIPLES OF THE DESIGN OF PRIMERS OR INCIATORS

INITIATOR MANUAL DESIGN

PRIMERS DESIGN TO AMPLIFY DNA

VIRTUAL PRACTICE WITH THE NCBI AND BLAST

 

DAY 3

THE COMPONENTS OF THE PCR

PRACTICAL PRIMER DESIGN USING THE BETA GLOBIN, ACTIN AND ABCB5 GENE

PRIMERS DESIGN TO AMPLIFY cDNA and RETRO TRANSCRIPT

PRIMERS DESIGN WITH PROGRAM

DAY 4

WORKSHOP WITH RESULTS

ANALYSIS AND REPORT OF PCR AMPLIFICATIONS OR AMPLICONS

GENOMIC DNA AMPLIFICATION

AMPLIFICATION TYPE RT-PCR

REAL TIME PCR

Do you want to know more about the agenda? Download the PDF version

What will you learn

Agarose gels

DNA

RNA

Design by PRIMERS

OPINIONS

Coming soon  your

"I really liked the tools and skills they taught us"

OFFER  from 1,800.00 MN (90 US dollars) to $ 1,350 MN (65.7 US dollars).

Pago PRIMERs
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